MGI, a subsidiary of BGI Group, unveiled its novel CoolNGS chemistry, a disruptive new sequencing approach for its DNBseq™ sequencing technology that enhances the throughput, accuracy, read length and cost effectiveness of DNA sequencing.
A global leader in innovative sequencing platforms, MGI is committed to developing and producing advanced genetic sequencing instruments, consumables and reagents and fostering the industry’s progress. CoolNGS was announced at the Advances in Genome Biology and Technology (AGBT) 2019 conference in Florida, where MGI scientific leaders delivered several talks.
At the conference, independently generated data from the DNBseq™ platform showed the instruments to be flexible and user friendly, with the base quality of sequencing data very high, read coverage evenly distributed, and very little GC bias.
Michael Quail, senior staff scientist – R&D Sequencing at the Wellcome Sanger Institute in the UK, described the DNBseq™ sequencing data in an abstract for his AGBT talk. The data “shows evenness of coverage and contiguity to be superior to that of its competitors,” he stated.
The novel proprietary CoolNGS sequencing chemistry announced today avoids DNA “scars” that can accumulate with traditional sequencing methods and affect the accuracy of subsequent reads. CoolNGS introduces unlabeled nucleotides and four fluorescent labeled antibodies in its cPAS (combinatorial probe anchored synthesis) sequencing process to recognize the incorporated bases. In this new process, the natural scarless bases are added in each sequencing cycle, enabling more accurate and longer reads.
CoolNGS also offers advantages in sensitivity and cost. “The multiple fluorescent dye molecules attached to the antibodies provide a higher signal-to-noise ratio and reduced consumption of expensive materials, together with incorporating natural bases with no interference between sequencing cycles,” said Dr. Radoje Drmanac, Chief Scientific Officer of MGI. “That’s what makes it so cool and natural for our DNBseq™, the most efficient high-throughput sequencing technology.”